Do you have broad experience within cellular reagents? Do you see yourself in a mentor role towards other scientists by providing your expertise within the area? Do you have experience in Development of stable cell lines utilising traditional, viral and Precise Genome Editing techniques? If you also have a positive, goal oriented and problem solving attitude then you might be the one we are looking for! The assignment is a consultant assignment placed at AstraZeneca in Gothenburg. It is planned to start 2016-10-01 and proceed until 2017-09-30.

The Arena

You will join the Discovery Sciences organization, working within a first-class scientific environment to support drug discovery. The Reagents and Assay Development department (RAD) of Discovery Sciences, is responsible for the generation of novel cellular reagents and assays to support projects in all phases of the drug discovery pipeline. We are seeking a Senior Research Scientist to join our cellular RAD team in Gothenburg Sweden, where we focus on delivering reagents and assays primarily for Cardiovascular and Metabolic diseases and Respiratory and Autoimmunity.

The role

To provide strong expertise in the competency of Cellular Reagent Generation and with a focus on cell line generation using traditional and precise genome editing approaches for use in cellular assays, target identification and target validation. To devise, champion and develop scientific areas aligned with project needs. To be an authority in the arena of cell science and precise genome editing, to lead this from a lab-based perspective and make significant contributions to the practical delivery of project goals. To coach and support more junior scientists by advising and challenging them and by providing expert advice to aid interpretation of complex data. To provide disciplinary scientific leadership and represent the department at internal interdisciplinary meetings in addition to external meetings and to publish novel science in high impact journals. To propose experimental strategies, troubleshoot problems and make significant contributions to the practical delivery of projects in compliance with AstraZeneca corporate responsibility policies and relevant safety standards.

It is essential to have good social and communication skills, be collaborative and a strong team player. Candidates should be able to work independently, take own initiatives and have a positive, goal oriented and problem solving attitude. Good organisational skills are required and you should be comfortable in presenting your results. Excellent English is required, both spoken and in writing.

Responsibilities

• To develop cellular reagents for target based approaches and phenotypic assays, target identification, target validation

• To design CRISPR-based strategies and tools required to generate cellular reagents to meet project needs

• To perform confirmation of cellular/genetic manipulation and functional validation of engineered cells

• To perform target identification and target validation studies in early projects

Highly desirable skills

• Development of stable cell lines utilising traditional, viral and Precise Genome Editing (CRISPR) techniques

• Transient and stable transfection utilising different technologies such as electroporation (Maxcyte, Nucleofector, Amaxa), lipid and PEI

• Knowledge of viral tools for expression and cell line generation

• Isolation of clonal cell lines (MACS, FACS, limiting dilution)

• Expert knowledge in the design and generation of Precise Genome Editing (CRISPR) strategies, tools and reagents required for cell line generation, target identification and target validation

• Genetic (ddPCR, NGS, AmpliconSeq) characterization of engineered cell lines

• Functional (phenotypic assays, ex

Timmar per vecka: Heltid Tillsvidare

Arbetsgivare Namn: Adecco Sweden AB

Arbetsgivare adress: Kungsgatan 60 10224 STOCKHOLM

Form av kontakt:
Frida Hallström titel : telefon 1: () telefon 2: () mobil: e-post: Frida.Hallstrom (at) adecco.se

Jobb kort beskrivning: Research and development managers

Land: Sweden

Område: Västra Götalands län

Avtalstid: Permanent

Typ av kontrakt: Full time

Källa till jobberbjudande: Public Employment Service of Sweden(AMS)

Hur man ansöker: Other - see free text